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5.
Optimization of Lipase-Catalyzed Synthesis of Betulinic Acid Ester
in Organic Solvents
Yamin Yasina*,
Mahiran Basrib,, Faujan Hj Ahmadb and Abu
Bakar Sallehc
a
International Education Centre, Universiti Teknologi MARA,
Kampus Seksyen 17, 40200 UiTM Shah Alam, Malaysia
bChemistry Department, , Faculty of Science, Universiti
Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
c Faculty of Biotechnology and Biomolecular Science,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
*Corresponding author. Tel. +03-55227038; Fax:
+03-55227065
E-mail:
yamin961@salam.uitm.edu.my
Abstract: Betulinic acid was
esterified with lipase as catalyst and chloroform as organic
solvent. Analysis of the product showed the esterification
occurred at C-3 position. The optimum reaction condition was
investigated. Enzymes, temperature, molar ratio, time of
reaction and amount of enzyme affected the yields of betulinic
acid ester produced. Novozyme was the best enzymes among other
enzymes tested that produced yields of 45%. The optimum yield
was produced at 12h reaction time, molar ratio of 4, temperature
of 50ºC and amount of enzyme of 150 mg.
Keywords: Esterification; Betulinic acid; Betulinic acid ester;
Lipase
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