Abstract: Ciprofloxacin (CPF), used in systemic and ophthalmic infections against gram-positive and gram-negative organism, is a quinolone derivative. In this study, ciprofloxacin-HCl (CPF-HCl) in tablets and ophthalmic solution was analyzed by using simple, fast and reliable zero-(A), first-(1D) and second- (2D) order derivative spectrophotometric methods. The quantitative determination of CPF-HCl was carried out using the A derivative values measured at 276 nm, 1D derivative values measured at 269 nm and 292 nm and the 2D derivative values measured at 277 nm. All developed methods gave similar and favorable results with respect to precision and accuracy, and the all RSD values are lower than 7.4 % and the recovery as a measure of the accuracy is close to 100 % in all cases. The developed A, 1D and 2D derivative spectrophotometric methods were applied to a pharmaceutical preparation as tablet form and ophthalmic solution which do not require any preliminary separation or treatment or derivatization of the samples. The results obtained from three spectrophotometric methods were compared and no significant difference was found statistically.
 

Keywords: Ciprofloxacin-HCl, Derivative Spectrophotometric Methods.