10.
Determination of Carbamazepine with a RP-HPLC Method in Human Plasma
Using Liquid–Liquid Extraction
Fatma Demirkayaa, Yucel Kadioglu1a
aDepartment of Analytical Chemistry, Faculty of Pharmacy, Ataturk
University, 25240, Erzurum, TURKEY
E-mail:
yucelkadi@hotmail.com or
yucel@atauni.edu.tr
Abstract:
Carbamazepine
(CBZ) is an antiepileptic drug, which are prescribed as first-line
drugs for the treatment of partial and generalized tonic-clonic
epileptic seizures. A simple and sensitive Reversed Phase High
Performance Liquid Chromatography (RP-HPLC) method for
quantification of CBZ in human plasma was developed and
validated. CBZ was extracted from human plasma with chloroform
with greater recovery than 91.7 % and separated using an
isocratic mobile phase, on a reversed-phase C18 column. The
effluent was monitored by UV detector at 220 nm and at a flow
rate of 1.0 mL/min. The linearity range of proposed method was
0.5-25 mg/mL. The intraday and inter-day relative standard
deviation and percent error values of the assay method were less
than 9.14 % and the method was found to be precise, accurate,
and specific during the study. The present method was selective
enough to analyze CBZ in human plasma without any tedious sample
clean-up procedure and was successfully applied for estimating
the pharmacokinetic parameters of CBZ following oral
administration of single dose of CBZ (Tegretol® CR 200 (200 mg))
to six healthy volunteers.
Key words:
Carbamazepine, pharmacokinetic, RP-HPLC.
<<< |