Abstract. The glycosides and aglycones of Thevetia peruviana seed were extracted, purified and characterised by infrared, 1H and 13C NMR spectroscopy. The aglycone of the major glycoside (Thevetin A) was converted to the acetate and was characterised. The 2,4-dinitrophenyl hydrazone derivative of the glycoside was also prepared and characterised. 2,4-dinitrophenyl hydrazine was found to be an effective spray reagent for the visualization of the thin layer chromatography of thevetia glycosides and aglycones.


KEYWORD: Glycosides, aglycones, Thevetia peruviana, NMR spectroscopy, thin layer chromatography