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10.
Estimation of Drotaverine Hydrochloride in Human Plasma
P. P. Dahivelkar*, S. B. Bari and S. J. Surana
Department of Pharmaceutical Analysis, R. C. Patel Institute of
Pharmaceutical Education and Research, Shirpur, India – 425 405
E-mail:
raj17579@rediffmail.com
Abstract.
A sensitive and specific liquid chromatography with ultraviolet
detection was developed for the estimation of drotaverine
hydrochloride (DH), in human plasma. Drotaverine hydrochloride
and internal standard diclofenac sodium were extracted from
plasma by a mixture of dichloromethane–isopropyl alcohol (80:20,
v/v). The compounds were separated on a Thermo BDS Hypersil C8
(250 X 4.6 mm, 5 mm, Thermo) column. The mobile phase containing
a mixture of acetonitrile and 0.02 M ammonium acetate buffer
(55:45) at pH = 3.5 was pumped at a flow rate of 1 mL min-1
with UV detection at 230 nm. Recovery from plasma was 98.68%.
KEY WORDS:Drotaverine
hydrochloride, Diclofenac sodium, Bio-analysis
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