Abstract. TLC (Thin layer chromatography) separation of sage (Salvia officinalis), lemonbalm (Melissa officinalis), thyme (Thymus vulgaris) and mountain tea (Sideritis perfoliata) was performed in this research. Essential oils and extracts of these drugs were loaded in various quantities to Kiesegel 60 glass plates using several solvents and different doses separately. Plant extracts were condensed under Nitrogen gas flow (3:1). Five different dyeing methods were used after achieving the reference plate. Guaiazulene (8 µl) and Liphophilic (10 µl) were used as references in every treatment. Their lay outs were investigated under visual and 254-366 nm UV light. Toluol:Ethylacetate (93:7) as solvent and Anisaldehyde-H2SO4 as dyeing material gave good separation and result.
 

Key words: TLC Separation, sage (Salvia officinalis), lemon balm (Melissa officinalis), thyme (Thymus vulgaris), mountain tea (Sideritis perfoliata)