19.
Rapid Prediction of Extractives Contents in Pinus caribaea Bark
Using Near Infrared Reflectance Spectroscopy
N. S.
A. Derkyi1,2*, B. Adu-Amankwaah2, D. Sekyere1.
and N. A. Darkwa3
1CSIR-Forestry Research Institute of Ghana, KNUST Box 63, Kumasi
2Department of Chemical and Petroleum Engineering, KNUST
3Faculty of Renewable Natural Resources, KNUST *E-mail:
dertch@csir-forig.org.gh
Abstract: The
potential of near infrared reflectance spectroscopy (NIRS) for
rapidly and accurately determining the extractives and
polyphenol contents in Pinus caribaea bark extracts was assessed.
Pinus caribaea bark samples were obtained from 110 trees in
plantation stands at different locations of Ghana and were then
scanned by NIRS. Their extractives and polyphenol contents
reference values were obtained by TAPPI T204 om-88 and Folin-Ciocalteau
methods respectively. These reference values were regressed
against different spectral transformations using partial least
square (PLS) regression. First derivative transformation
equation of the raw spectral data, resulted in a coefficient of
determination r2 in the external validation of 0.91 and 0.97
respectively for extractives content and polyphenol content. The
calibration samples covered a wide range of extractives content
from 34 – 45% and polyphenolic content from 16 – 23.5%. The
standard deviation to root mean square error of cross validation
ratio (SD/RMSECV), root mean square error of calibration to
standard deviation ratio (RMSEC/SD), RMSECV/RMSEC and r2 for
both extractives and polyphenol models were indicative of good
prediction equations. The predicted values were thus highly
correlated with time-consuming wet chemical measured values of
extractives content and polyphenol content. The use of NIRS for
the determination of the extractives and polyphenol contents in
Pinus caribaea bark thus provides an advantage of time saving
and cost of analysis.
Keywords: Modified Folin-Ciocalteau assay; Chemometric modeling;
Partial least square; soxhlet extractor; First derivative;
Bruker FT-IR spectrometer.
<<< |