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12.
On-Line Determination of Hydrochlorothiazide in Pharmaceutical and
Urine Samples After Preconcentration on Modified Polyurethane Foam
and UV Detection
Samah A. M. Alia*, S. M. Abdel Azeemb, M.F.
El-Shahatb
a Egyption Doping LAB.,
International Medical Center ,Ismallia Street, Egypt
b Chemistry Department, Faculty of Science, Fayoum University,
Fayoum City, Egypt
c Chemistry Department, Faculty of Science, Ain-Shams University,
Cairo, Egypt
Corresponding author Email:
samahali702@yahoo.com
Abstract:
A simple, sensitive
and fast method for determination of hydrochlorothiazide (HCT)
in pharmaceutics and urine samples with UV detection is
developed. The method
is based on the preconcentration of the analyte on a mini-column
packed with bromophenol blue grafted polyurethane foam (BPB-PUF)
via ion-pair complex.
The optimum preconcentration conditions were based on the
extraction of the analyte compound at pH 5.5,
preconcentration time 150 s and flow rate
2.0
mL min-1. Desorption of HCT was effected by passing
4 mL from
0.50 mol L−1 hydrochloric acid. The preconcentration
system provides a linearity range 2.0– 30 mg L-1,
limit of detection is 1.2 mg L-1 and limit of
quantification is 3.9 mg L-1.
The
developed approach provided an enrichment factor 21
and sample throughput 12 h-1. Method precision
expressed as relative standard deviation (RSD) was found to be 0.7
% for six replicate measurements (n=6) at concentration
level 5.0 mg L-1.
The method was successfully applied to the determination of HCT
in pharmaceutical and urine samples with recovery ranged from 91
to 107% and RSDs less than 2.5%.
Keywords:Hydrochlorothiazide,
preconcentration, bromophenol blue, polyurethane foam.
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