13- HPLC Analysis of Amino Acids on Octadecylsiloxane

Column Using a Nonionic Surfactant Mobile Phase

Majid Soleimani*¹/ Mohammad N. Sarbolouki² / Moslem Haghshenas³

¹Chemistry Department, Imam Khomeini International University, Qazvin, IRAN;

²Institute of Biochemistry and Biophysics, Tehran University, P. O. Box 13145-1384,

Tehran-IRAN; e-mail: sarbol@ibb.ut.ac,ir

³Engineering Research institute, P.O. Box 13445- 754, Tehran, IRAN

Correspondence author: ¹Chemistry Department, Imam Khomeini International University, P. O. Box 288, Postal Code 34194, Qazvin, IRAN e-mail: m-soleimani@hotmail.com

Abstract: In an attempt to provide a more convenient approach towards better resolution of amino acids by RP-HPLC, a nonionic surfactant in mobile phase was used. The effects of the structure and concentration of nonionic surfactant (Triton X-100), the pH, concentration of methanol or acetonitrile and the presence of anionic (SDS) or cationic (HTAB) surfactant in the mobile phase were studied. Twelve amino acids were successfully separated and detected (without the need for pre or post column derivatization) via reversed - phase chromatography.

Keywords: Amino acids, Triton X-100, HPLC Analysis, Reverse phase, Ion-pair chromatography

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